Effect of Different Ligand and Different Ligand Heterometal Xylaratohermanates on the Activity of α-L-Rhamnosidases Eupenicillium erubescens, Cryptococcus albidus and Penicillium tardum

α-L-Rhamnosidase [EC 3.2.1.40], enzyme of the hydrolase family has a wide range applications: in food industry, for example, winemaking to improve quality and aroma wines, production citrus juices drinks remove bitter components (naringin) that improves nutritional value these products; research as an analytical tool studying structure complex carbohydrate-substituted biopolymers. For successful use α-L-rhamnosidases various biotechnological processes, important aspect is development ways increase their activity. The main factors affecting growth metabolism microorganisms, including synthesis enzymes, are physicochemical conditions cultivation, composition nutrient medium, introduction substances raise yield enzyme, which manifested its At present, one priority directions modern study effect effector compounds capable modify studied enzymatic In this work, continuation previous studies, number mixed-ligand mixed-ligand-different-metal coordination germanium with xylaric acid (H5Xylar), 1,10-phenanthroline (Phen), 2,2-bipyridine (bipy) ions 3d-metals (Fe2+, Ni2+, Cu2+, Zn2+) were selected effectors. Study complexes on activity Eupenicillium erubescens, Cryptococcus аlbidus Penicillium tardum aim work. Methods. objects α-Lrhamnosidases from erubescens 248, albidus 1001, IMV F-100074. α-L-rhamnosidase was determined by Davis method using naringin substrate. We used 12 modifiers activity, established combination physical chemical methods: elemental analysis, thermogravimetry, IR spectroscopy X-ray structural analysis. Structures seven deposited Cambridge Crystallographic Database. When concentrations 0.1 0.01% used, exposure times 0.5 24 hours. test dissolved 0.1% dimethyl sulfoxide. UV-spectra absorption native modified preparations enzymes spectrophotometer-fluorimeter DeNovix DS-11 220–340 nm, concentration preparation 1.0 mg protein/mL. Results. Analysis totality obtained data (exposure time h, 0.1%) regarding E. C. P. showed influence varies depending producer strain. Our allow us present following series accordance different producers: еrubescens: < 11 5 3 4=10 1 8 2 6 7; albidus: 10 9 1=5 8=4 tardum: 12=2 4 7. Conclusions. results conclude compound (7)(-tris(bipyridine) nickel(II) μ-dihydroxyxylaratogermanate(IV)) most effective activator all three micromycete strains, (6)(tris(phenanthroline)nickel(II) − albidus, while (10)-(copper(II) μ-dihydroxyxylaratogermanate(IV)-cuprate(II)) only α-L-rhamnosidase.